Supplementary Figure 1: Gata1 expression in heamatopoietic stem and progenitor populations.
From: Distinct myeloid progenitor–differentiation pathways identified through single-cell RNA sequencing
(a) Unsupervised clustering according to 100 top variable genes across single pre-GM cells. The two main cell clusters are indicated. (b) List of genes differentially expressed between clusters A and B in (a). Gene IDs, P-values and false discovery rate (FDR) q-values are indicated. Genes were included if both P<0.001 and q<0.05. (c) Structure of the Gata1-EGFP transgene (not to scale). The Gata1 genomic locus was contained within the RP23-443E19 BAC. (d-e) Flow cytometry profiles and gating strategies for identification of pre-GM, GMP, pre-Meg-E, MkP, pre-CFU-E and CFU-E progenitors (d) and LSKCD150+Flt3− and LSKFlt3hi cells (e).
