Supplementary Figure 3: TIPARP-mediated regulation of type I IFN response to stimulation with viral mimetic nucleic acids. | Nature Immunology

Supplementary Figure 3: TIPARP-mediated regulation of type I IFN response to stimulation with viral mimetic nucleic acids.

From: Constitutive aryl hydrocarbon receptor signaling constrains type I interferon–mediated antiviral innate defense

Supplementary Figure 3

(a) HEK293T cells expressing control plasmid (Control) or TIPARP were treated with 3pRNA for the indicated time periods, and then assayed for IFNB1 and IFNA1 mRNA expression by qRT-PCR. n = 3 per group. (b) Luciferase activity of a p-125Luc reporter plasmid after 24 h of 3pRNA stimulation in HEK293T cells transfected with control plasmid (C) or the increasing doses of TIPARP expression vector (0.01, 0.1, and 1 μg). n = 3 per group. (c) Quantitative RT-PCR analysis of IL8 mRNA expression levels after 24 h of IL-1β stimulation in HEK293T cells transfected with control (C) or the increasing doses (0.01, 0.1, and 1 μg) of TIPARP expression vector. n = 3 per group. (d) Knockdown efficiency of siTIPARP-1 was evaluated by qRT-PCR analysis of TIPARP mRNA expression levels in HEK293T cells (left). Quantitative RT-PCR analysis of IFNB1 mRNA induction by 3pRNA stimulation in HEK293T cells treated with siControl or siTIPARP-1 (right). n = 3 per group. (e) Quantitative RT-PCR analysis of Ifna1 mRNA induction in response to the indicated viral MAMPs in Tiparp+/+ or Tiparp−/− MEFs. n = 3 per group. (f) Tiparp+/+ or Tiparp−/− BMDMs were stimulated with poly(rI:rC) without any transfection reagent for 24 h and IFN-β production was measured by ELISA. In this case, poly(rI:rC) was used as a ligand for TLR3. n = 3 per group. **P < 0.01 as compared with control (Student’s t-test). Data are representative of at least two independent experiments with similar results (mean ± s.d.). ND, not detected. NS, not significant.

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