Supplementary Figure 5: CD4+ T cells from CD3DH mice differentiate normally into TH1 cells.
From: TCR signal strength controls thymic differentiation of discrete proinflammatory γδ T cell subsets
(a) Flow cytometry of intracellular IFN-γ expression of WT (top) and CD3DH (bottom) TCRβ+CD4+ splenocytes incubated for 4 days without stimuli (left panels) or on plate-bound α-CD3ɛ and α-CD28 mAb (5ng/ml each), without (middle panels) or with (right panels) Th1-polarizing cytokines IL-2 (10 ng/ml) and IL-12 (50 ng/ml) plus anti-IL-4 (10 µg/mL) neutralizing antibody. Numbers indicate % of cells in each quadrant. Data are representative of two independent experiments.
