Supplementary Figure 1: γδ and αβ T cell development in CD3 mutant mice.

(a) Flow cytometry showing the CD3ɛ vs TCRδ phenotype of thymocytes from adult WT or CD3SH (single heterozygote for CD3δ or CD3γ) mice (n = 3 per group). Numbers within gated areas indicate % cells in each throughout. (b) Left, representative CD3ɛ vs TCRδ phenotype of thymocytes from the indicated adult mice. Numbers as in (a). Right, CD3ε and TCRδ expression (MFI) in gated TCRδ⁺ thymocytes and CD3⁺ thymocytes, respectively, from the indicated adult mice (n = 3 per group). Numbers indicate MFI (c) Gating strategy for CD3⁺ TCRδ⁺ γδ T cell identification. Doublets and dead cells were excluded. (d, e) Comparative analysis of TCRβ⁺CD1d⁺ NKT cells (d) and CD4⁺Foxp3⁺ Treg cells (e) in WT and CD3DH mice. Numbers in c, d, and e indicate percentage of cells within the marked region.