Supplementary Figure 1: Ogtfl/flLck-Cre+ mice have failed thymocyte development.
From: Glucose and glutamine fuel protein O-GlcNAcylation to control T cell self-renewal and malignancy
(a) Counts of TCRβ-expressing T cells in spleens (left) and inguinal LNs (right) from Ogtwt/wtLck-Cre+ (wt) and Ogtfl/Y or fl/flLck-Cre+ (KO) mice (n=8 each). ** P< 0.01 (Student's t-tests).
(b) MFI of Notch1, Notch2 and IL-7R analysed by flow cytometry is plotted for DN3, DN4 and DP subsets from OgtwtLck-Cre+ (wt) and Ogtfl/YLck-Cre+ (KO) mice. Mean and SD from staining of 3 mice is shown.
(c) CD25 and CD44 profiling of Thy1+ CD4-CD8- (DN) thymocytes from OgtwtLck-Cre+ (WT) and Ogtfl/YLck-Cre+ (KO) mice. Total cell numbers in each thymic DN subset are shown to the right (n=8 for WT and KO mice).
