Supplementary Figure 4: Analysis of global transcriptional differences between WT and Bach2−/– CD8+ T cells at day 7 after infection.
From: BACH2 regulates CD8+ T cell differentiation by controlling access of AP-1 factors to enhancers
a, Hierarchical clustering analysis of differentially expressed genes (p<0.05; log2 fold-change>1) between WT and KO OT-I cells isolated ex vivo 7 days following VV-OVA infection. Color scale shows FPKM values normalized to row maxima as indicated. b, Geneset enrichment analysis of the indicated geneset in global transcriptional differences between KO and WT cells. Statistical significance was tested using a weighted Kolmogorov–Smirnov test. FPKM values from 3 replicate RNA-Seq measurements per experimental condition are shown. c, Hierarchical clustering analysis of differentially expressed genes (p<0.05; log2 fold-change>1) between fractionated WT and KO CD62L− KLRG1− effector CD8+ T cells isolated ex vivo at day 7 following VV-OVA infection. Color scale shows average FPKM values normalized to row maxima as indicated. FPKM values from 2 replicate RNA-Seq measurements per experimental condition are shown.
