Supplementary Figure 6: Effect of BACH2 on chromatin accessibility and occupancy of Jun family AP-1 factors.
From: BACH2 regulates CD8+ T cell differentiation by controlling access of AP-1 factors to enhancers
a, Differences in JunD binding in WT and KO d5 in vitro activated CD8+ T cells do not correlate with differences in chromatin accessibility. Scatterplot comparing differences in JunD binding (y-axis) with differences in ATAC-Seq signal around BACH2 binding sites in WT and Bach2−/– d5 in vitro activated CD8+ T cells. Statistical significance was evaluated using the two-sample Kolmogorov-Smirnov test. b, Effect of BACH2 on binding of c-Jun, JunB and JunD. Enrichment of BACH2, c-Jun, JunB and JunD at indicated loci in d5 in vitro activated KO and WT CD8+ T cells relative to input. Enrichment values are normalized to WT cells. Bars and error (b) represent mean and s.e.m. of replicate measurements. *P<0.05; **P<0.01; ***P<0.005; ****P<0.001 (Student’s t-test).
