Supplementary Figure 4: Influence of treatment with collagenase and dispase on peritoneal-mast-cell transcription.
From: Expression profiling of constitutive mast cells reveals a unique identity within the immune system
Peritoneal mast cells were subjected to collagenase and dispase treatment for 30 min at 37°C, and microarray results were compared to untreated peritoneal mast cells. (a) Transcripts upregulated by two-fold or more in peritoneal mast cells relative to untreated mast cells following treatment with digestion enzymes. Colored gene symbols indicate fivefold (blue) or tenfold (red) higher expression. (b) Transcripts downregulated by twofold or more in peritoneal mast cells relative to untreated mast cells following treatment with digestion enzymes. Colored gene symbols indicate five-fold (blue) decreased expression. n = 6 untreated replicates and n = 3 enzymatically-treated replicates.
