Supplementary Figure 5: Influence of treatment with collagenase and dispase on peritoneal-mast-cell hierarchical clustering.
From: Expression profiling of constitutive mast cells reveals a unique identity within the immune system
Peritoneal mast cells were subjected to collagenase and dispase treatment for 30 min at 37°C, and microarray results were compared to all other analyzed immunocyte populations by hierarchical clustering using the top 15% most variable genes. Cell populations denoted using standard ImmGen abbreviations as indicated in Fig.1c. Bar height is inversely correlated to homology between linked populations. Data are from n = 3 independent experiments (skin, tongue, trachea, and enzymatically treated mast cells, spleen and blood basophils), n = 6 independent experiments from peritoneal mast cells, and n = 2 independent experiments from esophagus mast cells.
