Supplementary Figure 2: Gating strategies for the isolation of mast cells and basophils from peripheral tissue.

(a) Flow cytometric gating used to identify mast cells. Representative plots show skin mast cells. After excluding doublets, dead cells were excluded using propridium iodide and mast cells were identified as CD45⁺CD11c^‒CD11b^‒CD4^‒CD8^‒ CD19 ^‒ FcεR1α⁺CD117⁺. Arrows indicate sequential gates used. (b) Flow cytometric gating used to identify basophils. Representative plots show blood basophils. After excluding doublets, dead cells were excluded using propridium iodide and basophils were identified as CD4^‒CD8^‒CD19^‒NK1.1^‒CD117^‒CD49b⁺FcεR1α⁺. Arrows indicate sequential gates used.