Supplementary Figure 3: Validation of the expression changes in ILC2s stimulated with IL-1β.
From: IL-1 is a critical regulator of group 2 innate lymphoid cell function and plasticity
(a) The surface expression of HLA-DR/DP/DQ, CD80 and CD40L measured by flow cytometry in ILC2s freshly isolated from peripheral blood or cultured with IL-2 and IL-1β for 7 days. (b) mRNA levels of GATA3, RORC and RORA in freshly isolated ILC2s, CD56bright NK cells and ILC2s stimulated with IL-2 and IL-1β for 5 days. Data are normalized to GUSB and shown as relative values and represented as mean (± s.e.m.). *P<0.05, ** P < 0.01, *** P < 0.001 analyzed using one-way ANOVA followed by Turkey’s test (b). Data are representative of 6 experiments with similar results (a), or 6 (b) experiments with independent donors.
