Supplementary Figure 2: Regulation of TLR-induced expression of Cxcl1 and Il12b by Hes1 in BMDMs.
From: The transcriptional repressor Hes1 attenuates inflammation by regulating transcription elongation
(a,e) qPCR analysis of mRNA expression of Cxcl1 (a) or Il12b (e) in BMDMs from WT (Hey1+/+) and Hey1 KO (Hey1−/−) littermates, Hes1+/+Mx1-Cre (WT) and Hes1fl/flMx1-Cre (Hes1 KO) mice, or WT and DKO mice. BMDMs were untreated or stimulated with 100 ng/ml of LPS (a) or 10 ng/ml of LPS (e) for 3 h. (b) qPCR analysis of Hes1 mRNA expression in BMDMs from Hes1+/+Mx1-Cre (WT) and Hes1fl/flMx1-Cre (Hes1 KO) littermates. (c) Immunoblotting analysis of Hes1 protein expression in nuclear extraction of BMDMs from WT and Hes1 KO mice. TATA binding protein (TBP) served as a loading control. (d) qPCR analysis of Cxcl1 mRNA expression in WT and Hes1 KO BMDMs stimulated with Pam3Cys (10 ng/ml) or R848 (1 μg/ml) for 3 h. Representative data is shown as means ± s.d. of technical triplicate determinants from two independent experiments (a,d,e). Cumulative data from 9 independent experiments are shown in b (means and s.d.). ****P<0.0001 (two-tailed Student’s paired t-test).
