Supplementary Figure 3: Hes1-mediated suppression of Cxcl1 expression is independent of Cre type and deletion method.
From: The transcriptional repressor Hes1 attenuates inflammation by regulating transcription elongation
(a,d) qPCR analysis of mRNA expression of Hes1 in BMDMs from Hes1+/+Cre-ERT2 (WT) and Hes1fl/flCre-ERT2 (Hes1 KO) mice (a) or Hes1+/+Lyz2Cre (WT) and Hes1fl/flLyz2Cre (Hes1 KO) mice (d). (b) Immunoblotting analysis of Hes1 protein expression in nuclear extracts of BMDMs from Hes1+/+Cre-ERT2 (WT) and Hes1fl/flCre-ERT2 (Hes1 KO) mice. TATA binding protein (TBP) served as a loading control. (c,e) qPCR analysis of mRNA expression of Cxcl1 in Hes1+/+Cre-ERT2 (WT) and Hes1fl/flCre-ERT2 (Hes1 KO) mice (c) or Hes1+/+Lyz2Cre (WT) and Hes1fl/flLyz2Cre (Hes1 KO) mice (e) BMDMs stimulated with LPS (10 ng/ml) for 3 h (c) or indicated periods (e). Cumulative data from three independent experiments are shown in a (means and s.d). Representative data from at least two independent experiments are shown as mean and s.d. of technical triplicate determinants (c-e).*P<0.05 (two-tailed Student’s paired t-test).
