Supplementary Figure 7: Global Pol II–distribution patterns in wild-type and Hes1-deficient macrophages.
From: The transcriptional repressor Hes1 attenuates inflammation by regulating transcription elongation
(a,b) Heat map depiction of Pol II ChIP-seq reads in Hes1+/+Mx1-Cre (WT) and Hes1fl/flMx1-Cre (Hes1 KO) BMDMs untreated or stimulated with 10 ng/ml of LPS for 1 h. Genes longer than 750 bp are aligned around mouse RefSeq TSSs and ranked by decreasing Pol II ChIP-seq signals near TSSs (±150bp). Pol II distribution patterns of all genes and of LPS-induced genes are shown in (a) and (b) respectively. (c) Genes with enhanced Pol II occupancy on the gene body regions in Hes1-deficient BMDMs. The list was generated by calculating Pol II density throughout the gene body regions in Hes1 KO + LPS versus WT + LPS conditions. Data are obtained from two independent Pol II ChIP-seq experiments.
