Supplementary Figure 5: LPS-induced inflammasome activation in Pggt1b-deficient BMDMs depends on caspase-1 and Asc but not Nlrp3. | Nature Immunology

Supplementary Figure 5: LPS-induced inflammasome activation in Pggt1b-deficient BMDMs depends on caspase-1 and Asc but not Nlrp3.

From: Control of the innate immune response by the mevalonate pathway

Supplementary Figure 5

Immunoblots of IL-1β, caspase-1 in the supernatant and lysate of (a) Pggt1bfl/fl Lyz2-Cre, Pggt1bfl/fl Lyz2-Cre Casp-1−/−, Pggt1b+/+ Casp1+/+ or Casp-1−/− BMDMs stimulated with LPS or LPS and Nigericin; (b) Pggt1b+/+ Pycard+/+, Pycard−/−, Pggt1bfl/fl Lyz2-Cre, Pggt1bfl/fl Lyz2-Cre Pycard−/−, or BMDMs stimulated with LPS or LPS and Nigericin; (c) Pggt1b+/+ Nlrp3+/+, Pggt1bfl/fl Lyz2-Cre, Pggt1bfl/fl Lyz2-Cre Nlrp3−/−, or Nlrp3−/− BMDMs stimulated with LPS or LPS and Nigericin.

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