Supplementary Figure 5: Notch signaling promotes all-or-none Bcl11b activation in a dose-dependent manner.
From: Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment
Flow cytometry analysis of bone marrow derived Bcl11b-YFP- DN2 cells cultured under the indicated conditions with 5 ng/mL IL-7 and Flt3L for four days. CD25 versus Bcl11b-YFP levels (top), and histograms of Bcl11b-YFP and CellTrace Violet levels (bottom) are shown. Initial levels (Day 0) are shown as gray dotted lines. GSI: γ-secretase inhibitor (Calbiochem/EMD Millipore, Billerica, MA), a small molecule inhibitor of Notch signaling.
