Supplementary Figure 7: Effects of TCF-1 perturbation on Bcl11b activation in pro-T cells.
From: Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment
A) Flow cytometry analysis of bone marrow DN progenitors transduced with either shRNA to Tcf7 (shTcf7) or a non-targeting control (shRandom), then cultured on plates coated with 6 μg/mL DL1 with 5 ng/mL SCF, IL-7 and Flt3L for four days. Levels of the dendritic cell marker CD11c against FSC (top), as well as CD25 levels versus Bcl11b-YFP levels (middle) or Bcl11b-YFP level distributions (bottom) for CD11c- populations are shown. Knockdown of Tcf7 promotes DC trans-differentiation in DN2 cells, but does not impede Bcl11b activation. B) Flow cytometry analysis of Bcl11b-YFP expression levels in bone marrow DN progenitors transduced with a control or VEX-GFP retroviral construct for over-expression of TCF-1 (encoded by Tcf7), and cultured on OP9- or OP9-DL1 monolayers in 5 ng/mL IL-7 and Flt3L for three days. TCF-1 over-expression does not affect Bcl11b activation from either ETP or DN2 progenitors.
