Supplementary Figure 8: Runx1, but not TCF-1 or GATA-3, controls Bcl11b expression amplitude in mature T cells.
From: Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment
A) Published transcriptomic data on Bcl11b expression in wildtype or Tcf7-/- DN3 thymocytes9 (left), or in wildtype or Gata3-/- Th2 cells10 (right). Levels of Bcl11b expression are similar in the absence or presence of either factor. B) Flow cytometry analysis of CD4 and CD8 splenic T-cells activated with anti-TCRb and anti-CD28 for 1 day, transduced with shRNA constructs, and cultured for 3 days. Plots show CD8 versus CD3 for cells transduced with retrovirus (top), along with Bcl11b level distributions for gated non-CD8 T-cell populations (bottom). Corresponding data for CD8 T-cells are shown in Fig. 8. Solid black lines indicate cells transduced with random shRNA, and dotted lines indicate background levels from non-fluorescent T-cells. Data from (A) represent mean and S.D. of two independent experiments.
