Supplementary Figure 1: Binding profiles of key transcription factors to the Bcl11b gene and enhancer.
From: Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment
A)-C) UCSC genome browser tracks (mm9 coordinates) showing published ChIP-Seq binding data of the following transcription factors to the Bcl11b gene: Notch11, CSL1, Gata32, PU.12, TCF-13, Runx14 (both WT and Runx1-/- control) and Ikaros5. The H3K4me2 histone mark is also included2. The primary cell types on which ChIP-Seq was performed are also indicated. A) Global view of Bcl11b locus, showing gene body in relation to distal enhancer and downstream gene desert. Shaded yellow bars indicate gene and enhancer locations. B) Bcl11b gene body, and C) Bcl11b enhancer domain. Gray shaded bars indicate regions containing clusters of transcription factor binding sites and H3K4me marks. Asterisk indicates binding sites tested in enhancer transfection assays6. Note that a high resolution version of this figure is also provided separately.
