Supplementary Figure 2: CD1a suppresses contact hypersensitivity mediated by IFN-γ-producing cells.

Wild-type (WT) and CD1a-tg mice (n = 3 per group) were sensitized with 0.5% DNFB by painting on the abdomen on day 0, and challenged with either 0.2% DNFB or vehicle (veh) on ears on day 5 after sensitization. (a) Ear swelling after challenge. (b) Frequencies of Gr-1^hiCD11b^hi granulocytes (left panel), CD4⁺ or CD8⁺ T cells among live CD45⁺TCRβ⁺ cells (middle panel), and IL-17A⁺ or IFN-γ⁺ cells among TCRβ⁺ cells (right panel). (c) Absolute cell numbers of CD8⁺ or CD4⁺ ab T cells, IFN-γ- or IL-17A-producing cells in ears. * P < 0.05, ** P < 0.01, *** P < 0.001, using unpaired t-test. Results are representative of three independent experiments (mean ± s.e.m).