Supplementary Figure 2: FAMIN localizes to peroxisomes.
From: C13orf31 (FAMIN) is a central regulator of immunometabolic function
(a) Co-localization by immunofluorescence (IF) of FAMIN (red) with PMP70 (green) in primary human macrophages (MΦ). DAPI nuclear staining, blue; scale bar = 10 μm. White box corresponds to enlarged images shown in Fig. 1d. (b–e) Co-localization by IF of FAMIN (red) with PMP70 (green), catalase (green), cytochrome oxidase IV (green) and calreticulin (green) in THP-1 macrophages. DAPI, blue; scale bar = 10 μm for original images and 5 μm for enlarged images. Upper panels: original image, middle panels: enlargement of the area shown in the white box, lower panels: further enlargement as indicated. No significant co-localization of FAMIN was detected with CENP-A, centromere; caveolin-2, cholesterol/sphingolipid enriched plasma membrane; EEA1 and RAB5, early endosome; LAMP1, lysosomes; NUP98, nuclear envelope; fibrillin, nucleolus and syntaxin 6, trans-Golgi network (data not shown). (f) Proximity-ligation assay (PLA) of FAMIN in combination with PMP70, Catalase (red) or centromere protein A (red) as negative control in THP-1 macrophages. DAPI, blue; scale bar = 10 μm. White box corresponds to enlarged images shown in Fig. 1e. Data are representative of three independent experiments.
