Supplementary Figure 5: FAMIN does not directly affect the Krebs cycle.
From: C13orf31 (FAMIN) is a central regulator of immunometabolic function
(a) Schematic representation of 1,2-13C-glucose incorporation, via citrate, into fatty acyl species. (b) Basal extracellular acidification rate (ECAR) of mFamin–/– and mFamin+/+ M1 and M2 macrophages (MΦ) in the presence of exogenous pyruvate (n = 5/14). (c) Relative levels of malate, fumarate, succinate and α-ketoglutarate in M1 and M2 macrophages. (d) Oxygen consumption rate (OCR) of mFamin–/– and mFamin+/+ M2 macrophages treated as indicated with 40 μM etomoxir (ETO) 1 h prior to OCR measurement and followed by sequential treatment (dotted vertical lines) with oligomycin (Oligo), FCCP, and rotenone plus antimycin (Rot + ant). *P < 0.05, **P < 0.01 (Unpaired, two-tailed Student’s t-test). Data are pooled from three independent experiments (b; mean ± S.E.M.), from one experiment with seven mice per group (c; mean ± S.E.M.) or from one experiment with three mice representative of two independent experiments (d; mean ± S.E.M.).
