Supplementary Figure 1: The PGRP-LC locus encodes a subset of isoforms containing an alternative first exon.
From: The regulatory isoform rPGRP-LC induces immune resolution via endosomal degradation of receptors
(a) Clustal Omega multiple sequence alignment of 5’ and 3’ regions of exon 5 in 11 sequenced Drosophila species indicate a conserved Kozak sequence upstream of the potential translation start site, and a splice donor site in 3’.
(b) RT-qPCR amplification of rLC isoforms from DNase-treated total RNA from the white1118 strain, reverse-transcribed using oligo dT primers, shows that exon 5 encoding the rLC cytosolic domain is spliced to all ectodomain-encoding exons (x, a and y isoforms). See also Fig. 1c for details of PGRP-LC locus architecture. Sequenced products were blasted against the UCSC Genome Browser D. melanogaster Apr. 2006 (BDGP R5/dm3) Assembly. Note that FlyBase lists only the rLC isoform containing the x ectodomain, referred to as PGRP-LC-RE.
(c) PCR products as sequenced in b.
