Supplementary Figure 2: Particle-induced NLRP3 inflammasome activation is partially suppressed by PGE2, and cyclooxygenase inhibition blocks NLRP3 activation.
From: NLRP3 inflammasome inhibition is disrupted in a group of auto-inflammatory disease CAPS mutations
(a-c) BMDM were primed with LPS. (a,b) The indicated concentration of PGE2 was added 5 min before stimulation with (left) silica (250 μg/ml for 5 h) or (right) MSU (150 μg/ml for 4 h). (c) Indomethacin (50 μM) was added 30 min before stimulation with nigericin (5 μM for 30 min). (a,c) Total IL-1β release in cell supernatants analyzed by ELISA. (b,c) Immunoblot analysis of culture supernatants (SN) and lysates (LYS). *P < 0.005 (unpaired two-tailed t test). (a-c) Data are from one experiment representative of three separate experiments with similar results, (a-c) BMDM were derived from 2 mice per experiment mixed together, (a,c) three replicates, each replicate is one well + s.e.m.
