Supplementary Figure 8: The Tat-Epep is specific to infection with RNA viruses.
From: Infection-specific phosphorylation of glutamyl-prolyl tRNA synthetase induces antiviral immunity
(a–c) Tat-Epep has no significant effect on virus replication (a) or IFN-β (b) and IL-6 (c) secretion in RAW264.7 infected with HSV-GFP (MOI = 1) for 12 h. HSV-GFP-infected RAW264.7 cells treated with PBS were used as negative controls. (d) Viability of RAW264.7 cells as measured in an MTS assay after treatment with the indicated doses of Tat-Epep for 12 h. (e) Viability of 293T cells after treatment with Tat-Epep for 12 or 24 h. Ctrl, 293T cells treated with a lytic detergent (digitonin, 30 μg/ml) for 15 min as a positive control. Results are expressed as the mean ± SD of two independent biological replicates incorporating triplicate samples.
