Supplementary Figure 1: EPRS expression in multiple cell lines upon viral infection.
From: Infection-specific phosphorylation of glutamyl-prolyl tRNA synthetase induces antiviral immunity
(a,b) EPRS is slightly induced upon viral induction. qPCR of Eprs mRNA (a) and immunoblot analysis of corresponding endogenous EPRS expression (b) in multiple cell lines, including C57/B6 mouse-derived BMDM, U937, RAW264.7, A549, and 293T cells infected with PR8-GFP or VSV-GFP. (c) qPCR analysis of representative Isg mRNA expression under the same conditions as in (a). (d) qPCR analysis of Eprs mRNA in U937 and RAW264.7 cells treated with IFN-β (1000 units/ml). Isg15 mRNA was analyzed as a control. (e) qPCR analysis of Eprs mRNA in RIG-I-sufficient (Ddx58+/+) or RIG-I-deficient (Ddx58-/-) MEF cells infected with VSV-GFP. Data are representative of two (a-e) independent biological replicates with similar results (mean and s.d. of triplicate in a,c-e).
