Supplementary Figure 6: The phenotype and function of TFC cells.
From: CXCR5+ follicular cytotoxic T cells control viral infection in B cell follicles
CD45.1+ P14 cells were adoptively transferred into congenically marked (CD45.2) wildtype mice, which were then infected with LCMV (DOCILE). The expression of indicated proteins in naïve (grey), CXCR5+ TFC (red), CXCR5- non-TFC (blue), B220+ B (black) cells at day 8 p.i.
(a) The expression of ICOSL on TFC cells.
(b) IL-21-GFP reporter mice were infected with LCMV Docile and the expression of IL-21-GFP in indicated populations were analysed at day 15 p.i..
(c) Ex vivo killing of LCMV GP33-41 peptide-pulsed non-TFH or TFH SMARTA cells, or B cells by CXCR5+ TFC or CXCR5− non-TFC P14 cells purified at day 8 p.i.
Each symbol represents one mouse (A, B and D) or one sample (C), bars represent means. GMFI: geometric mean fluorescence intensity. **P < 0.01 (Mann-Whitney's U-test). Data are representative of two independent experiments.
