Supplementary Figure 1: In vitro and in vivo model systems used in these studies and measurement of in vitro caspase-8 activity.
From: Glioma-induced inhibition of caspase-3 in microglia promotes a tumor-supportive phenotype
(a) Diagram illustrating a transwell segregated microglia-glioma coculture setup to study glioma’s influence over microglia biology. (b) Measurement of LETDase (caspase-8) activity in BV2 microglia cells cultured in medium alone or segregated coculture with a panel of glioma cells at 6h (black bars) and 24h (white bars) after coculture. Results are presented relative to those of BV2 microglia cultured in medium alone, set as 1. (c) Illustration of how postnatal day 16-17 male C57/BL6/J mice were injected intrastriatally with syngeneic GFP-expressing GL261 glioma cells. One or two weeks after glioma injection, mice were sacrificed and brain tissue processed for analysis. Statistics and error bars: mean ± s.d. n=6 (b) of biological replicates. Data was analyzed using two-tailed Student’s t-test. ns, not significant (P > 0.05); *P< 0.05; **P< 0.01.
