Supplementary Figure 1: Gene expression profile of CD4⁺ T cells and CTL responses in Bcl6-deficient mice.

(a) Gene expression profile in the resting CD4⁺ T cells were analyzed by an Affymetrix microarray chip (MouseGenome 430 2.0 array). Differential expressions were found in 10 genes that indicated. (b) Gene expression profile of OT-II derived CD4⁺ T cells in the response to OVA. The heat map represents expression of signature genes of T_H1, T_H2, T_FH, and T_H17 subsets. (c) Flow cytometry analysis of NP tetramer binding CD8⁺ T cells. Spleen cells were obtained from PR8-infected WT and Bcl6^ΔT mice at day10 post infection and were cultured with NP peptide pulsed EL-4 cells in presence of IL-2. After 5-day culture, cells were stained with NP tetramer and anti-CD8a mAb. The graph shows the percentage of NP tetramer binding CD8 cells in WT and Bcl6^ΔT mice (n=4). (d) For the killing assay, NP peptide pulsed EL-4 cells were labeled with CFSE and then co-cultured with spleen cells. After 6hr-culture, killing rate was determined with the EL-4 cell number. The percent lysis was calculated based on the background lysis of EL-4 cells without NP peptide. Mean of the specific lysis were indicated at different effector/target ratios (n=4). Error bars represent S.D.