Supplementary Figure 4: Transgenic expression of Glut1 results in altered metabolic and immune phenotypes in T_reg cells.

a-d. CD4⁺CD25^- T cells were isolated from the spleens of control and Glut1-Tg mice and polarized under T_reg skewing conditions. Cells were examined for (a) Glut1 expression levels by immunoblot, (b) glucose uptake, (c) ROS production as measured by DCFDA and (d) ECAR and OCR levels were measured using the Seahorse Extracellular Flux Analyzer before and after the addition of the specified inhibitors. e-f. CD4⁺Foxp3⁺ T cells from the spleens of control and Glut1-Tg mice were examined for expression of (e) CD25 and (f) Helios proteins. Data are representative of three independent experiments (a-b, d, e) or compiled data from three independent experiments (c, f). Means and standard deviations are shown, * p<0.05.