Supplementary Figure 5: Transgenic Glut1 expression alters the immunological phenotype of T_reg cells.

a-f. CD4⁺CD25^- T cells were isolated from the spleens of control and Glut1-Tg mice and polarized under T_reg skewing conditions. Skewed cells were analyzed for (a) Foxp3 expression by flow cytometry, (b) cell size by FSC analysis, (c) CD25 and (d) ICOS protein expression by flow cytometry. (e) RNA expression levels of a panel of immunosuppressive related genes by QPCR and (f) percentage of IFNγ and IL-2 producing CD4⁺Foxp3⁺ cells by flow cytometry are shown. Data are representative of three independent experiments (a-d) or the average of six biological replicates (e) or four biological replicates (f). Means and standard deviations are shown, * p<0.05.