Supplementary Figure 5: Additional analysis of altered metabolic pathways in TFR cell–suppressed B cells.
From: Suppression by TFR cells leads to durable and selective inhibition of B cell effector function
(a) Glut1 staining in B cells for experiments shown in Fig. 4b. (b) (left) Gating of division number for experiments shown in Fig. 4c. (right) IgG1+ staining in B cells gated by division number. (c) IgG1+ staining in B cells that were added to 3 day cultures and harvested 20 hours later as in Fig. 4d. (d) Glutamine uptake measured from culture supernatants from cultures as in Fig 4b. CD4+ICOS–CXCR5–FoxP3+ Treg cells were added in some conditions. (e) IgG1+ B cells in culture supernatants from cultures as in (a) with the addition of glutaminolysis inhibitor BPTES.
