Supplementary Figure 4: Identification of plasma cells and plasmablasts. | Nature Immunology

Supplementary Figure 4: Identification of plasma cells and plasmablasts.

From: Regulation of autoantibody activity by the IL-23–TH17 axis determines the onset of autoimmune disease

Supplementary Figure 4

(a) Sorting strategy and sorting purity analysis of splenic plasma cells (CD19+CD138+B220-) and plasmablasts (CD19+CD138lowB220+) for mRNA-analysis. (b) Gating strategy for the flow cytometric analysis of St6gal1 protein expression in splenic Ova-specific plasmacells and plasmacells isolated during collagen-induced arthritis. Plasmacells were gated to be B220lowCD138+Taci+ and Ova+, respectively.

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