Supplementary Figure 2: Humoral immune response in wild-type and Il23a−/− mice during CIA. | Nature Immunology

Supplementary Figure 2: Humoral immune response in wild-type and Il23a−/− mice during CIA.

From: Regulation of autoantibody activity by the IL-23–TH17 axis determines the onset of autoimmune disease

Supplementary Figure 2

(a-d) Analysis of levels of (a) collagen type II (CII)-specific IgG, (b) CII-specific IgG subsets, (c) IgG specifically directed against murine CII (d) and IgG detecting different murine CII epitopes in the sera of WT and Il23a−/− mice at day 50 after induction of collagen-induced arthritis (CIA). (e) KSCN titration assay for the determination of the binding affinity of CII-specific IgG in WT and Il23a−/− mice. (f,g) Analysis of the appearance of (f) germinal centers and the appearance of germinal center (CD19 pre-gated) B cells (g) at day 26 after induction of CIA in WT and Il23a−/− mice. (h) Measurement of the levels of regular and high affinity antibodies in response to a T cell dependent immunization with 4-hydroxy-3-nitrophenylacetyl (NP) coupled to chicken g-globulin (NP-CGG) in WT and Il23a−/− mice. Error bars represent SEM*P<0.05; **P<0.01; ***P<0.001; Student’s t test.

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