Supplementary Figure 4: Characterization of iNKT cell–specific Fasl–/– chimeras.
From: Neutrophils license iNKT cells to regulate self-reactive mouse B cell responses
(a) Flow cytometry data showing congenic marker expression (CD45.1+ vs CD45.2+) of cells from mixed BM chimeras generated by transfer of Traj18–/– + wild-type or Traj18–/– + Fasl–/– BM into Traj18–/– recipients. (b) Flow cytometry data showing gating strategy for iNKT cells in spleens of mixed BM chimeras discussed in (a). Congenic markers are used to identify lymphocytes derived from Traj18–/– (CD45.1+; left panels) vs wild-type or Fasl–/– (CD45.2+; right panels) BM within the same mouse. (c) Flow cytometry histograms showing expression of FasL in iNKT cells (top) and CD8+ T cells (bottom), gated as in (b). Filled histograms indicate expression in Fasl–/– cells; red histograms indicate expression in wild-type cells. (d) Flow cytometry data showing proportions of iNKT cells in chimeric mice reconstituted with wild-type [iNKT(WT FasL)] or Fasl–/– iNKT cells [iNKT(ΔFasL)]. *P < 0.05 (two-tailed unpaired Student’s t-test [(d; n = 4 per group). Data represents three independent experiments (a-d). Box height indicates group mean + s.e.m.
