Supplementary Figure 5: Gene expression profile of Sall1-deficient microglia.

(a-c) Gene expression analysis of microglia sorted from Sall1^CreER/fl and Sall1^fl/fl mice on day 1 after 5 times of tamoxifen treatment every second day as described in Figure 4. (a) Venn diagram of differentially expressed genes. (b) Volcano plot showing log₂ ratios vs. p values (log₁₀) of all 12,673 detected genes. Genes with highest significance values are annotated. (c) Expression (log₂ ratio) of Sall1-regulated genes clustered according to their indicated GO-pathways; IS, Immune system; (bold indicates genes discussed in Results). (d) Multiplex immunoassays show levels (pg/mg) of IL-1, IL-6, TNF-α and IL-10 in serum and whole tissue lysates of spleen, kidney and liver of untreated (control) mice and Sall1^CreER/fl and Sall1^fl/fl mice at day 6 after start of tamoxifen treatment. (e) Graph displays cell counts of DCX⁺ neuroblasts in hippocampal brain sections of tamoxifen treated Cx3cr1^CreERSall1^fl/fl and Cre⁻ (control) littermates; each symbol represents an individual mouse; small horizontal lines indicate the mean (± s.e.m.). * p = 0.0006 (unpaired t-test). Data are representative of 3-5 mice pooled per genotype and biological replicate, 3 experiments (a-c); 3 (Sall1^CreER/fl, Sall1^fl/fl), 2 (untreated) mice, 1 experiment (d; mean ± s.e.m.); 3 (Cx3cr1^CreERSall1^fl/fl), 4 (control) mice, 2 experiments (e).