Supplementary Figure 6: RNF128 positively regulates antiviral response.
(a) VSV titers and VSV-G protein in HEK293 (2×105) transfected with the V5-RNF128-WT, mutant V5-RNF128-H2N2 and V5-RNF128-△PA expression plasmid and then infected with VSV (MOI, 0.1). (b) VSV titers and VSV-G protein in mouse peritoneal macrophages transfected with control siRNA or RNF128 siRNA and then infected with VSV (MOI, 0.1). (c) HSV-1 genomic DNA and titers in mouse primary macrophages transfected with control siRNA or RNF128 siRNA and then infected by HSV-1 (MOI, 10). (d) VSV titers and VSV-G protein in THP-1 cells transfected with control siRNA or RNF128 siRNA and then infected with VSV (MOI, 0.1). (e) HSV-1 titers and genomic DNA in THP-1 cells transfected with control siRNA or RNF128 siRNA and then infected by HSV-1 (MOI, 10). *p <0.05, **p <0.01 (two-tail Student's t-test). Data are from three independent experiments (a-e; mean±S.D. of triplicate assays) or are representative of three independent experiments with similar results (a,b,d).
