Supplementary Figure 3: Exposure to air pollutants induces expression of Tslp and Il33 in keratinocytes. | Nature Immunology

Supplementary Figure 3: Exposure to air pollutants induces expression of Tslp and Il33 in keratinocytes.

From: The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin

Supplementary Figure 3

(a) Knockout efficiency determined via PCR of the Ahr gene using isolated epidermal samples of Ahrfl/fl mice and Ahrfl/flKrt5-Cre mice (dots represent individual mice, 5 mice each). (b, c) Relative expressions of Tslp, Il33 and Cyp1a1 in mouse primary keratinocytes exposed to different concentrations of benzo[a]pyrene (B[a]P; 3 independent samplings; b) and in 1 µM B[a]P-exposed mouse primary keratinocytes with or without AhR knockdown (3 independent samplings, c). (d) Relative expression of CYP1A1 in HaCaT cells exposed to FICZ (5 independent samplings). (e) Relative expression of TSLP, IL33, FLG and CYP1A1 in differentiated or undifferentiated HEKns exposed to DMBA or FICZ (6 independent samplings). Data in a - d, coral lines represent the mean. Data in e, box-and-whisker plot (line, median; box, 25th to 75th percentiles; bar, minimum to maximum). Data in a-e, unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001.

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