Supplementary Figure 4: Evaluation of CYP1A1 expression and hyper-innervation in human skin.

(a) Representative immunostaining for CYP1A1 in the specimens classified as –, + and ++. Images from the same experiments as in Fig. 7g and Supplementary Table 1. –, negative or positive only in basal layer; +, positive in less than the half of the epidermis; ++, positive in more than the half of the epidermis. Immunostaining for TSLP in the same specimens are presented in the lower panels. Scale bars, 100 µm. (b) Immunostaining for neuronal marker PGP9.5 in skin samples of normal subjects, AD patients, and contact dermatitis patients. Dotted line, the epidermis-dermis boundary; scale bar, 20 µm; representative images of 3 individuals each.