Supplementary Figure 2: Influence of skin-barrier destruction and cytokine expression on AhR-CA mouse phenotypes. | Nature Immunology

Supplementary Figure 2: Influence of skin-barrier destruction and cytokine expression on AhR-CA mouse phenotypes.

From: The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin

Supplementary Figure 2

(a) OVA uptake by MHC class II+ (MHCII+) antigen-presenting cells at the cortex of regional lymph nodes. Scale bar, 20 µm; 3 mice each. (b) Number of OVA+ and OVA+MHCII+ cells observed in a. (c-e) Relative mRNA expressions of Ephb2 (c), Artn (d), and St3gal3 (e). The expressions of Ephb2 and St3gal3 were evaluated in the RT-qPCR analysis of epidermal samples from neonatal AhR-CA mice compared with their expression in samples from wild-type littermates (5 mice each). Artn expression was examined in the skin from WT and AhR-CA mice with or without nail-clipping (6 mice each). (f) Relative expression of AD-related genes at the rostral back after Artemin-neutralization (4 mice each). (g-i) Appearance (g, Scale bar, 10 mm), number of scratching behaviors in 10 min (h) and alloknesis score (i, lines represent the median; Mann-Whitney U test) of 4 Tslp-KO, 5 AhR-CA, and 6 AhR-CATslp-KO compound mice. Data in b-f, and h, dots represent individual mice; lines represent the mean; unpaired t-test; *p < 0.05 and **p < 0.01.

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