Supplementary Figure 2: Analysis of CD4 and CD8 T cell development in wild type and Tet2-3 DKO mice.
From: TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells
(a) Thymic cellularity in wild type (n=9) and Tet2-3 DKO (n=10) mice at 3-4 weeks of age.
(b) Top, Analysis of DN, DP, CD4 and CD8 SP thymic subsets defined by surface staining for CD4 and CD8 markers. Bottom, Representation of these subsets after gating out iNKT cells as defined by 
GalCer-CD1d and TCRβ staining.
(c) Percentage and (d), Number of DP, CD4 SP and CD8 SP cells in the thymus of WT (n=6) and Tet2-3 DKO (n=5) mice at 3-4 weeks.
(e) Representation of CD4 and CD8 T cells in lymph nodes (LN, top) and spleen (bottom) of WT and Tet2-3 DKO mice at 3-4 weeks.
(f) Total number of cells in lymph nodes (LN) of 3-4 week old WT (n=3) and Tet2-3 DKO (n=3) mice.
(g) Percentage and number of CD4 and CD8 cells in the LN of WT (n=6) and Tet2-3 DKO (n=3) mice analyzed at 3-4 weeks old.
(h) Percentage and number of CD4 and CD8 cells in the spleen of WT (n=6) and Tet2-3 DKO (n=3) mice analyzed at 3-4 weeks old.
Data are mean ± SEM. *P<0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001 (unpaired t test).
At least 3 independent experiments were performed.
