Supplementary Figure 3: Profound loss of TET proteins is required for dysregulated expansion and function of iNKT cells. | Nature Immunology

Supplementary Figure 3: Profound loss of TET proteins is required for dysregulated expansion and function of iNKT cells.

From: TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells

Supplementary Figure 3

(a) iNKT cells in the thymus of representative 20 day-old WT versus Tet2-/-, Tet3 KO and Tet2-3 DKO mice, defined by staining with GalCer-CD1d tetramer and anti-TCRβ.

(b) Increased percentages (left) and numbers (right) of iNKT cells in spleens isolated from 4 week-old WT (n=6), T2-/- (T2 KO, n=3), T3 KO (n=3) and Tet2-3 DKO (n=10) mice.

(c) Histogram evaluating the expression of RORγt (left); PLZF (center); and CD4 (right) in WT, single Tet2-/- (Tet2 KO), Tet3 KO, Tet2-3 DKO thymic iNKT cells.

(d, e) Histogram evaluating the expression of RORγt in the spleen (d) and lymph nodes (e) of WT, single Tet2-/-, Tet3 KO, Tet2-3 DKO iNKT cells.

Data are mean ± SEM. *P<0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001 (unpaired t test)

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