Supplementary Figure 4: Cytokine production in Tet2-3 DKO iNKT cells. | Nature Immunology

Supplementary Figure 4: Cytokine production in Tet2-3 DKO iNKT cells.

From: TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells

Supplementary Figure 4

(a) Representative flow cytometric analysis of IL17 secretion by GalCer-CD1d tetramer+ TCRβ+ cells.

(b) Percentage of IL17-secreting iNKT cells among total iNKT cells.

(c) Percentage of IL17-secreting iNKT cells among total thymocytes.

(d) Representative flow cytometric analysis of IFNγ secretion by GalCer-CD1d tetramer+ TCRβ+ TCR cells.

(e) Percentage of IFNγ-secreting iNKT cells among total iNKT cells.

(f) Percentage of IFNγ-secreting iNKT cells among total thymocytes.

(g) Representative flow cytometric analysis of IL4 secretion by GalCer-CD1d tetramer+ TCRβ+ cells.

(h) Percentage of IL4-secreting iNKT cells among iNKT cells.

(i) Percentage of IL4-secreting iNKT cells among total thymocytes.

In all cases (a-i) 3 mice per genotype were evaluated in 2 independent experiments.

(j) CD8 SP thymocytes were assessed by flow cytometry for the expression of markers that characterize memory-like CD8 cells: surface markers CD122, CD44, CXCR3 and the transcription factor Eomes.

(k) Percentage of CD8 SP thymocytes that express CD122, CD44, CXCR3 and Eomes. WT (n=3) and Tet2-3 DKO (n=3) mice. For (j) and (k) 3 independent experiments were performed.

Data are mean ± SEM. *P<0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001 (unpaired t test)

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