Supplementary Figure 3: In vivo priming of T cell responses in the absence of IL-6-producing DCs.
From: Trans-presentation of IL-6 by dendritic cells is required for the priming of pathogenic TH17 cells
DC conditional IL-6 reporter mice (CD11c-Cre x Il6RD/wt) were immunized with MOG(35-55) in CFA followed by control treatment (mouse IgG2a isotype) or anti-Thy1.1 (19E12) to deplete IL-6 (Thy1.1)+ DCs. Antibody treatment was performed by i.p. injection of 200 μg antibody every other day starting on day 1 after immunization. On day 7 after immunization, draining lymph nodes (LN) and spleen (SPL) were prepared and stained for Foxp3 to quantify the fraction of Tregs in the CD4+ T cell compartment (a). (b) Subsequent to PMA/ionomycin restimulation, LN CD4+ T cells were stained intracellularly for IL-17, GM-CSF, IFN-γ, and IL-10. (c, d) Antigen specific T cell responses were assessed by intracellular staining of CD40L (CD154) and cytokines in splenic CD4+ T cells of control-treated or IL-6+ DC-depleted mice after recall with MOG(35-55). Mean + SD (n=5 mice per genotype).
