Supplementary Figure 7: IL-6 cluster signaling and surface and intracellular expression of IL-6Rα and gp130 by CD11b+ DCs.
From: Trans-presentation of IL-6 by dendritic cells is required for the priming of pathogenic TH17 cells
(a) Scheme of IL-6 cluster signaling. IL-6 is loaded onto the IL-6Rα in intracellular compartments of DCs and is brought to the cell membrane as an IL-6-IL-6Rα complex. During a cognate interaction between DCs and T cells, DCs present IL-6 via their IL-6Rα in trans to T cells. Trans-presentation of IL-6 leads to the engagement of gp130 on the T cell side (IL-6 cluster signaling) and induces a pathogenic phenotype in sensitized T cells. (b) CD11b+ DCs express IL-6Rα on their cell surface. WT mice were immunized with MOG(35-55) in CFA and on day 7 after immunization, cells from draining lymph nodes (LN) and spleen (SPL) were analyzed by flow cytometry. (b) IL-6Rα and gp130 expression was assessed in CD11c+MHC class II+CD11b+ cDC2 either by surface staining (left column) or by intracellular staining (right column). Grey: isotype. Blue overlay: IL-6Rα or gp130, respectively. (c) Surface expression of IL-6Rα was assessed on splenic cDC1 cells (CD103+) or CD11b+ DCs isolated from immunized mice on day 7 after immunization.
