Supplementary Figure 4: TRIM31 targets MAVS. | Nature Immunology

Supplementary Figure 4: TRIM31 targets MAVS.

From: The ubiquitin E3 ligase TRIM31 promotes aggregation and activation of the signaling adaptor MAVS through Lys63-linked polyubiquitination

Supplementary Figure 4

(a) Luciferase activity in HEK293T cells transfected with IFNB1 luciferase reporter and expression plasmids for RIG-IN, MDA5, MAVS, TBK1, IKKɛ and IRF3 5D together with Flag-TRIM31 expression plasmid for 24 h. (b) Luciferase activity in HEK293T cells transfected with control siRNA or TRIM31 siRNA and IFNB1 luciferase reporter or ISRE luciferase reporter together with expression plasmids for RIG-IN, MAVS and TBK1 for 24 h. (c) Coimmunoprecipitation analysis of Flag-TRIM31 interaction with Myc-IRF3 or Myc-STING in HEK293T cells. The data are representative of 3 independent experiments with three biological replicates (means ± S.D. in a, b). *p < 0.01. (two-tailed Student’s t-test).

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