Supplementary Figure 1: TRIM31 is recruited to mitochondria after infection with SeV.

(a) Confocal microscopy of TRIM31-GFP transfected into HEK293T cells for 24 h followed with SeV infection for 6 h. MitoTracker (red) were used to label mitochondria. Scale bar 10 μm. (b) Immunoblot of TRIM31 protein and TOMM20 protein in the crude mitochondrial extracts (upper) and TRIM31 protein and β-actin in the whole cell lysate (lower) prepared from peritoneal macrophages infected with SeV for indicated times. (c) Immunoblot of TRIM31 protein in mouse peritoneal macrophages infected with SeV for indicated times. (d) qPCR analysis of Trim31, Trim26, Trim30α, Ifnb1 expression in primary peritoneal macrophages transfected with mice TRIM31 siRNA, TRIM26 siRNA, TRIM30α siRNA or control siRNA for 48 h, followed with SeV infection for indicated times. mRNA results are presented relative to those of untreated cells transfected with control siRNA. (e) qPCR analysis of TRIM31 and IFNB1 mRNA in THP-1 cells transfected with human TRIM31 siRNA or control siRNA for 48 h, followed with SeV infection for indicated times. mRNA results are presented as in d. (f) qPCR analysis of IFNB1 mRNA in Hela cells transfected with Flag-TRIM31 expression plasmid or control vector for 24 h, followed with SeV infection or transfection with poly(I:C) for the indicated times. mRNA results are presented relative to those of untreated cells transfected with control plasmid. The data are representative of 3 independent experiments with three biological replicates (means ± S.D. in d-f). *p < 0.01. (two-tailed Student’s t-test).