Figure 1: The expression and gene targeting of Themis2.
From: Themis2 lowers the threshold for B cell activation during positive selection
(a,b) mRNA expression of Themis2 (a) and Themis1 (b) in flow-sorted cells from wild-type (WT) mice. (a) Themis2 expression in BM pro/pre (B220+CD43+IgM−IgD−) and immature (B220+CD43−IgM+IgD−) B cells, peritoneal B1 (B220loCD19+IgM+IgD−) B cells, splenic follicular (B220hiCD23+CD21+) and marginal zone (B220hiCD23−CD21hi) B cells, and germinal center (B220+GL7+CD95+) and IgG1 (B220+IgG1+) B cells following immunization with SRBC. (b) Themis1 expression in thymic cell populations. Columns show means from triplicate sorts and qPCR with maximum and minimum measurements. (c) Relative expression of Themis2 splenic mRNA across indicated exon junctions. Bars show means with 95% confidence limits and comparison by unpaired t test. *P < 0.01, **P < 0.001. (d) Representative immunoblot analysis of Themis2 and β-actin protein expression in splenocytes from mice carrying wild-type, heterozygote (Themis2+/−) and homozygote (Themis2−/−) targeted alleles of Themis2 stained with a rabbit anti-Themis2 antibody14.
