Figure 2: Themis2 is not required for the generation of a pre-immune B cell repertoire, but does reduce BCR expression on mature B cells. | Nature Immunology

Figure 2: Themis2 is not required for the generation of a pre-immune B cell repertoire, but does reduce BCR expression on mature B cells.

From: Themis2 lowers the threshold for B cell activation during positive selection

Figure 2

(a) Number of B cells in six wild-type and five Themis2−/− mice, gated on transitional (B220hiCD23−CD21lo), follicular (B220hiCD23+CD21+) and MZ (B220hi CD23−CD21hi) B cells in the spleen. Columns show means and s.e.m. (b) Serum IgM antibody titers in 9 wild-type and 21 Themis2−/− adult mice, aged 8–14 weeks. Bars show means and 95% confidence limits. (c) Flow cytometry of wild-type and Themis2−/− B220+ BM B cells showing the frequency and gating of pro/pre (IgM−IgD−), immature (IgM+IgD−) and mature (IgM+IgD+). Histograms are representative of more than ten mice. (d) Mean cell surface IgM expression on B220+IgD+ wild-type and Themis2−/− B cells in BM and spleen, expressed as mean fluorescent intensity (MFI) by flow cytometry and gated as in c. Bars show means and 95% confidence limits and comparison by unpaired t test. *P < 0.05, ***P < 0.001. Data are representative of results from three independent experiments.

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