Figure 3: Reduced germinal center and class-switched B cells in the absence of Themis2.
From: Themis2 lowers the threshold for B cell activation during positive selection
(a) Flow cytometry of wild-type and Themis2−/− splenic B220+ B cells in naive mice and in mice 8 d after intraperitoneal (IP) immunization with SRBCs showing the gating and typical frequency of GC (B220+GL7+CD95+) B cell numbers. Data are representative of more than 12 mice in 3 independent experiments. (b) Number of GC (B220+GL7+CD95+) B cells (top) and class-switched (B220+IgG1+) B cells (bottom) in wild-type and Themis2−/− mice, 8 d after immunization with SRBCs, with bars showing means and s.e.m. Data are representative of results from three independent experiments. (c) IgM and IgG1 anti-NP25 antibody titers in WT and Themis2−/− mice 7 and 14 d after primary immunization with NP3-CGG. Symbols represent individual mice, bars means and 95% confidence limits and comparison by unpaired t test. *P < 0.05, **P < 0.01 and ***P < 0.001
